Human NTXI (Cross Linked N-telopeptide of Type I Collagen) ELISA Kit
The Human NTXI (Cross-linked N-telopeptide of Type I Collagen) ELISA Kit is specifically designed for the precise measurement of NTXI levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.NTXI is a key marker for bone resorption and is involved in bone turnover and remodeling processes. Elevated levels of NTXI have been associated with various bone-related disorders, including osteoporosis and osteoarthritis, making it a valuable tool for studying these conditions and developing therapeutic interventions.With its accuracy and reliability, the Human NTXI ELISA Kit is an essential tool for researchers investigating bone health and diseases related to bone metabolism.
Product Name:
Human NTXI (Cross Linked N-telopeptide of Type I Collagen) ELISA Kit
SKU:
HUES01970
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
