The Human OLFM4 (Olfactomedin 4) ELISA Kit is meticulously crafted for the precise and quantitative detection of Olfactomedin 4 levels in various human biological samples. OLFM4, a key protein associated with cell differentiation, epithelial integrity, and stem cell regulation, plays a significant role in various physiological processes and disease conditions. Accurate measurement of OLFM4 levels is essential for unraveling its impact on cellular activities and pathophysiological mechanisms. Our OLFM4 ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results crucial for your research or clinical studies.
Manufactured under strict quality control measures, this kit provides robust performance and user-friendly procedures, making it an excellent choice for a wide range of applications. Rely on Assay Genie's OLFM4 ELISA Kit for accurate and consistent quantification of this important biomarker in your investigations.
Product Name:
Human OLFM4 (Olfactomedin 4) ELISA Kit
SKU:
AEES00136
Target:
Human OLFM4 (Olfactomedin 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OLFM4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OLFM4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OLFM4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OLFM4. You can calculate the concentration of Human OLFM4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-100
95-104
89-95
Average (%)
97
99
92
1:4
Range (%)
91-106
96-103
94-105
Average (%)
99
100
100
1:8
Range (%)
91-104
87-95
92-100
Average (%)
98
91
97
1:16
Range (%)
88-98
88-99
85-98
Average (%)
93
94
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-96
91
EDTA plasma (n=5)
95-108
103
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.94
22.03
99.62
8.91
21.66
82.2
Standard deviation
0.59
0.99
5.22
0.51
1.01
3.73
C V (%)
5.94
4.49
5.24
5.72
4.66
4.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human OLFM4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OLFM4 in samples. No significant cross-reactivity or interference between Human OLFM4 and analogues was observed.
