The Human OPN (Osteopontin) CLIA Kit is a cutting-edge tool for the precise quantification of osteopontin levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Osteopontin is a key protein involved in various biological processes, including tissue remodeling, immune response modulation, and cell adhesion. Its dysregulation has been implicated in numerous diseases, such as cancer, inflammation, and fibrosis, underscoring its importance as a biomarker in disease research and therapeutic development.
The Human OPN CLIA Kit from Assay Genie provides researchers with a robust and reliable platform for investigating the role of osteopontin in health and disease. With its ease of use and proven performance, this kit is an invaluable asset for advancing scientific understanding and driving medical innovations.
Product Name:
Human OPN (Osteopontin) CLIA Kit
SKU:
HUES00783
Target:
Human OPN (Osteopontin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OPN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human OPN. You can calculate the concentration of Human OPN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
102-120
86-99
Average (%)
101
110
91
1:4
Range (%)
85-98
93-108
99-111
Average (%)
90
101
105
1:8
Range (%)
98-113
90-104
102-118
Average (%)
106
96
108
1:16
Range (%)
103-116
92-103
88-102
Average (%)
109
98
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
92
EDTA plasma (n=5)
92-105
97
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
209.13
490.86
1620.53
189.29
459.49
1517.49
Standard deviation
19.26
54.73
122.67
18.65
49.62
148.26
C V (%)
9.21
11.15
7.57
9.85
10.8
9.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human OPN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OPN in samples. No significant cross-reactivity or interference between Human OPN and analogues was observed.
