Human PAI1 (Plasminogen Activator Inhibitor 1) ELISA Kit
The Human PAI-1 (Plasminogen Activator Inhibitor-1) ELISA Kit is a reliable tool for the precise measurement of PAI-1 levels in human biological samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees accurate and reproducible results, making it suitable for a variety of research applications.PAI-1 is a key regulator of fibrinolysis, playing a critical role in blood clotting and thrombosis. Dysregulation of PAI-1 has been linked to various diseases including cardiovascular disorders, obesity, and diabetes, highlighting its importance as a biomarker for studying these conditions and exploring potential therapeutic interventions.Get your Human PAI-1 ELISA Kit today and unlock new insights into the role of PAI-1 in health and disease.
Product Name:
Human PAI1 (Plasminogen Activator Inhibitor 1) ELISA Kit
SKU:
HUES03013
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
