Human PGF2 alpha (Prostaglandin F2 alpha) ELISA Kit
The Human PGF2-alpha (Prostaglandin F2-alpha) ELISA Kit is specially designed for the accurate measurement of PGF2-alpha levels in human samples like serum, plasma, and cell culture supernatants. This advanced kit offers high sensitivity and specificity, ensuring precise and reproducible results that are crucial for various research applications.PGF2-alpha is a key prostaglandin involved in various physiological processes such as smooth muscle contraction, inflammation, and reproduction. It plays a vital role in conditions like asthma, inflammation, and reproductive disorders, making it a valuable biomarker for understanding these diseases and exploring potential therapeutic interventions.
With the Human PGF2-alpha ELISA Kit, researchers can confidently quantify PGF2-alpha levels in human samples, leading to a deeper understanding of the role of this bioactive lipid and its implications in different disease states. Trust this kit for accurate and reliable results that can drive groundbreaking research in biomedical and pharmaceutical fields.
Product Name:
Human PGF2 alpha (Prostaglandin F2 alpha) ELISA Kit
SKU:
HUES02795
Target:
Human PGF2 alpha (Prostaglandin F2 alpha)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGF2α. During the reaction, PGF2α in the sample or standard competes with a fixed amount of PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGF2α. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGF2α in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
93-111
84-96
Average (%)
106
101
90
1:4
Range (%)
90-105
93-106
98-115
Average (%)
96
99
105
1:8
Range (%)
87-102
86-102
100-114
Average (%)
94
93
107
1:16
Range (%)
84-94
93-109
96-112
Average (%)
89
100
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.3
47.5
205.1
21.9
47.7
224.4
Standard deviation
1.3
2.8
10.9
1.5
2.5
7.4
C V (%)
5.35
5.89
5.31
6.85
5.24
3.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of PGF2α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes PGF2α in samples. No significant cross-reactivity or interference between PGF2α and analogues was observed.
