The Human PKM2 (Pyruvate Kinase, Muscle) ELISA Kit is a specialized assay designed for the precise quantitative analysis of Pyruvate Kinase M2, also known as PKM2, in various biological samples. PKM2 is a critical enzyme involved in glycolysis regulation and metabolic processes essential for cell function and energy production.
Accurate measurement of PKM2 levels is crucial for understanding its role in metabolic pathways, cell proliferation, and differentiation. This ELISA kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under strict quality control standards, the PKM2 ELISA Kit delivers robust performance while maintaining ease of use, making it an excellent choice for both research and clinical applications. Researchers can trust the accuracy and dependability of Assay Genie's PKM2 ELISA Kit for quantifying this vital biomarker in their studies.
Product Name:
Human PKM2 (Pyruvate Kinase, Muscle) ELISA Kit
SKU:
AEES00099
Target:
Human PKM2 (Pyruvate Kinase, Muscle)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 IU/mL
Detection range:
15.63-1000 IU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PKM2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PKM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PKM2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PKM2. You can calculate the concentration of Human PKM2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
91-103
84-98
Average (%)
93
95
92
1:4
Range (%)
88-101
86-98
96-103
Average (%)
93
90
100
1:8
Range (%)
89-98
96-111
93-106
Average (%)
95
104
98
1:16
Range (%)
99-107
97-105
92-102
Average (%)
104
101
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
95
EDTA plasma (n=5)
98-110
105
Cell culture media (n=5)
90-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.47
116.17
471.44
47.83
121.5
409.39
Standard deviation
2.69
5.66
22.06
2.5
5.67
17.19
C V (%)
5.67
4.87
4.68
5.23
4.67
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human PKM2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PKM2 in samples. No significant cross-reactivity or interference between Human PKM2 and analogues was observed.
