Human PPA1 Recombinant Protein (RPPB2149)
- SKU:
- RPPB2149
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q15181
- Research Area:
- Enzymes
Description
| Product Name: | Human PPA1 Recombinant Protein |
| Product Code: | RPPB2149 |
| Size: | 20µg |
| Species: | Human |
| Target: | PPA1 |
| Synonyms: | Pyrophosphatase (inorganic) 1, PP, PP1, IOPPP, SID6-8061, Pyrophosphate phospho-hydrolase, PPase, cytosolic inorganic pyrophosphatase, diphosphate phosphohydrolase, inorganic diphosphatase, EC 3.6.1.1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PPA1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 2mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMSGFST EERAAPFSLE YRVFLKNEKG QYISPFHDIP IYADKDVFHM VVEVPRWSNA KMEIATKDPL NPIKQDVKKG KLRYVANLFP YKGYIWNYGA IPQTWEDPGH NDKHTGCCGD NDPIDVCEIG SKVCARGEII GVKVLGILAM IDEGETDWKV IAINVDDPDA ANYNDINDVK RLKPGYLEAT VDWFRRYKVP DGKPENEFAF NAEFKDKDFA IDIIKSTHDH WKALVTKKTN GKGISCMNTT LSESPFKCDP DAARAIVDAL PPPCESACTV PTDVDKWFHH QKN |
PPA1 is a member of the PPase family. PPA1 catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. Many biochemical pathways use the hydrolysis of PPi to two phosphate ions to make the reactions permanently irreversible. A prominent illustration of this phenomenon is the inorganic pyrophosphatase catalyzation in the hydrolysis reaction at the beginning of lipid degradation. Inorganic pyrophosphatase provides the motivation for the activation of fatty acids destined for oxidation by stimulating the rapid hydrolysis of pyrophosphate.
PPA1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 313 amino acids (1-289) and having a molecular mass of 35.2kDa.PPA1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PPA1: is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Hydrolase; EC 3.6.1.1; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 10q11.1-q24 Cellular Component: cytoplasm; cytosol Molecular Function:inorganic diphosphatase activity; magnesium ion binding Biological Process: tRNA aminoacylation for protein translation; gene expression; phosphate metabolic process |
| NCBI Summary: | The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15181 |
| NCBI GenInfo Identifier: | 8247940 |
| NCBI Gene ID: | 5464 |
| NCBI Accession: | Q15181.2 |
| UniProt Secondary Accession: | Q15181,Q2M348, Q5SQT7, Q6P7P4, Q9UQJ5, Q9Y5B1, |
| UniProt Related Accession: | Q15181 |
| Molecular Weight: | 289 |
| NCBI Full Name: | Inorganic pyrophosphatase |
| NCBI Synonym Full Names: | pyrophosphatase (inorganic) 1 |
| NCBI Official Symbol: | PPA1�� |
| NCBI Official Synonym Symbols: | PP; PP1; IOPPP; HEL-S-66p; SID6-8061�� |
| NCBI Protein Information: | inorganic pyrophosphatase; PPase; pyrophosphatase 1; inorganic diphosphatase; inorganic pyrophosphatase 1; diphosphate phosphohydrolase; pyrophosphate phospho-hydrolase; cytosolic inorganic pyrophosphatase; epididymis secretory sperm binding protein Li 66p |
| UniProt Protein Name: | Inorganic pyrophosphatase |
| UniProt Synonym Protein Names: | Pyrophosphate phospho-hydrolase; PPase |
| Protein Family: | Soluble inorganic pyrophosphatase |
| UniProt Gene Name: | PPA1�� |
| UniProt Entry Name: | IPYR_HUMAN |
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