Human PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma) CLIA Kit
The Human PPAR Gamma (Peroxisome Proliferator-Activated Receptor Gamma) CLIA Kit is a powerful tool for the accurate detection of PPAR Gamma levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PPAR Gamma is a key nuclear receptor involved in the regulation of lipid metabolism, glucose homeostasis, and inflammation.
Dysregulation of PPAR Gamma is associated with metabolic disorders such as obesity, diabetes, and cardiovascular disease, making it a valuable target for therapeutic interventions.With the Human PPAR Gamma CLIA Kit, researchers can easily measure PPAR Gamma levels in human samples, providing valuable insights into metabolic pathways and potential treatment strategies for metabolic diseases. Trust in this kit for accurate and precise results in your research endeavors.
Product Name:
Human PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma) CLIA Kit
SKU:
HUES00793
Target:
Human PPAR-gamma (Peroxisome Proliferator Activated Receptor Gamma)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PPAR-γ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PPAR-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PPAR-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PPAR-γ. You can calculate the concentration of Human PPAR-γ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
86-96
95-111
Average (%)
109
91
102
1:4
Range (%)
84-98
90-101
96-107
Average (%)
91
96
101
1:8
Range (%)
89-104
89-99
98-110
Average (%)
95
94
104
1:16
Range (%)
88-100
97-111
96-111
Average (%)
94
103
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
99-114
107
Cell culture media (n=5)
98-111
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.4
250.93
744.92
109.91
231.81
677.71
Standard deviation
9.41
24.94
48.2
13.22
27.72
77.12
C V (%)
9.01
9.94
6.47
12.03
11.96
11.38
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human PPAR-γ concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human PPAR-γ in samples. No significant cross-reactivity or interference between Human PPAR-γ and analogues was observed.
