Description
| Product Name: | Human RAC2 Recombinant Protein |
| Product Code: | RPPB4379 |
| Size: | 20µg |
| Species: | Human |
| Target: | RAC2 |
| Synonyms: | p21-Rac2, Small G protein, GX, RAC2, HSPC022, EN-7. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The RAC2 protein solution (1mg/ml) contains 1x PBS pH-7.4. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMQA IKCVVVGDGA VGKTCLLISY TTNAFPGEYI PTVFDNYSAN VMVDSKPVNL GLWDTAGQED YDRLRPLSYP QTDVFLICFS LVSPASYENV RAKWFPEVRH HCPSTPIILV GTKLDLRDDK DTIEKLKEKK LAPITYPQGL ALAKEIDSVK YLECSALTQR GLKTVFDEAI RAVLCPQPTR QQKRACSLL |
RAC2 is a small signaling G protein/GTPase which is part of the RAS superfamily of small GTP-binding proteins which regulate cell growth, actin cytoskeletal organization, cell proliferation, survival, cell cycle progression, gene transcription rgulation and the activation of protein kinases. RAC2 cycles between an active gtp-bound and inactive gdp-bound state. In active state, RAC2 binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 is involved in the regulation of the nadph oxidase.
Recombinant RAC2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 419 amino acids and having a molecular mass of 47 kDa. RAC2 is fused to GST and purified by conventional chromatography techniques.
| UniProt Protein Function: | RAC2: Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Augments the production of reactive oxygen species (ROS) by NADPH oxidase. Interacts with DOCK2, which may activate it. Hematopoietic specific. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase. Belongs to the small GTPase superfamily. Rho family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Rho; Motility/polarity/chemotaxis; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: actin filament; cytosol; focal adhesion; lamellipodium; phagocytic vesicle membrane; plasma membrane Molecular Function:GTP binding Biological Process: actin filament organization; platelet activation; regulation of hydrogen peroxide metabolic process; regulation of small GTPase mediated signal transduction; signal transduction Disease: Neutrophil Immunodeficiency Syndrome |
| NCBI Summary: | This gene encodes a member of the Ras superfamily of small guanosine triphosphate (GTP)-metabolizing proteins. The encoded protein localizes to the plasma membrane, where it regulates diverse processes, such as secretion, phagocytosis, and cell polarization. Activity of this protein is also involved in the generation of reactive oxygen species. Mutations in this gene are associated with neutrophil immunodeficiency syndrome. There is a pseudogene for this gene on chromosome 6. [provided by RefSeq, Jul 2013] |
| UniProt Code: | P15153 |
| NCBI GenInfo Identifier: | 131806 |
| NCBI Gene ID: | 5880 |
| NCBI Accession: | P15153.1 |
| UniProt Secondary Accession: | P15153,Q9UDJ4, |
| UniProt Related Accession: | P15153,AAB22207 |
| Molecular Weight: | 21,429 Da |
| NCBI Full Name: | Ras-related C3 botulinum toxin substrate 2 |
| NCBI Synonym Full Names: | ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2) |
| NCBI Official Symbol: | RAC2�� |
| NCBI Official Synonym Symbols: | Gx; EN-7; HSPC022; p21-Rac2�� |
| NCBI Protein Information: | ras-related C3 botulinum toxin substrate 2 |
| UniProt Protein Name: | Ras-related C3 botulinum toxin substrate 2 |
| UniProt Synonym Protein Names: | GX; Small G protein; p21-Rac2 |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAC2�� |
| UniProt Entry Name: | RAC2_HUMAN |
