Human REV1 Recombinant Protein (RPPB2242)
- SKU:
- RPPB2242
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9UBZ9
- Research Area:
- Enzymes
Description
| Product Name: | Human REV1 Recombinant Protein |
| Product Code: | RPPB2242 |
| Size: | 20µg |
| Species: | Human |
| Target: | REV1 |
| Synonyms: | REV1 Polymerase (DNA Directed), REV1L, Alpha Integrin-Binding Protein 80, Rev1-Like Terminal Deoxycytidyl Transferase, AIBP80, REV1 (Yeast Homolog)- Like, REV1 Homolog (S. Cerevisiae) , REV1-Like (Yeast) , DNA Repair Protein REV1, REV1 Homolog, REV1- Like, EC 2.7.7.-. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The REV1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGVAIYVNGY TDPSAEELRK LMMLHGGQYH VYYSRSKTTH IIATNLPNAK IKELKGEKVI RPEWIVESIK AGRLLSYIPY QLYTKQSSVQ KGLSFNPVCR PEDPLPGPSN IAKQLNNRVN HIVKKIETEN EVKVNGMNSW NEEDENNDFS FVDLEQTSPG RKQNGIPHPR GSTAIFNGHT PSSNGALKTQ DCLVPMVNSV ASRLSPA |
REV1 Polymerase (REV1) contains a BRCT domain, which is imperative in protein-protein interactions. REV1 is a deoxycytidyl transferase involved in DNA repair. REV1 assists in the 1st step in the bypass of abasic lesions by the incorporation of a nucleotide opposite the lesion. REV1 is essential for normal generation of mutations by physical and chemical agents. REV1 transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. A role for the human Rev1-like protein has been suggested as a scaffold which recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA.
REV1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (51-256) and having a molecular mass of 25.2kDa.REV1 is fused to a 21 amino acid His-tag at N-terminus.
| UniProt Protein Function: | Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. |
| NCBI Summary: | This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016] |
| UniProt Code: | Q9UBZ9 |
| NCBI GenInfo Identifier: | 84043967 |
| NCBI Gene ID: | 51455 |
| NCBI Accession: | NP_001032961.1 |
| UniProt Secondary Accession: | Q9UBZ9,O95941, Q53SI7, Q9C0J4, Q9NUP2, |
| UniProt Related Accession: | Q9UBZ9 |
| Molecular Weight: | Observed: 138 kDaPredicted: 139 kDa |
| NCBI Full Name: | DNA repair protein REV1 isoform 2 |
| NCBI Synonym Full Names: | REV1, DNA directed polymerase |
| NCBI Official Symbol: | REV1�� |
| NCBI Official Synonym Symbols: | REV1L; AIBP80�� |
| NCBI Protein Information: | DNA repair protein REV1 |
| UniProt Protein Name: | DNA repair protein REV1 |
| UniProt Synonym Protein Names: | Alpha integrin-binding protein 80; AIBP80; Rev1-like terminal deoxycytidyl transferase |
| Protein Family: | DNA repair protein |
| UniProt Gene Name: | REV1�� |
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