The Human S100A8 ELISA Kit is a highly reliable and sensitive assay designed to accurately measure levels of S100A8 protein in human serum, plasma, and cell culture supernatants. This kit is essential for research applications involving inflammatory responses, as S100A8 is a key protein involved in the regulation of inflammation and immune responses. S100A8 is a member of the S100 protein family, known for its role in modulating inflammatory processes and immune responses. Elevated levels of S100A8 have been associated with a variety of inflammatory diseases, including rheumatoid arthritis, inflammatory bowel disease, and psoriasis.
This ELISA kit provides researchers with a powerful tool for studying the role of S100A8 in these conditions and potential therapeutic interventions. With its high sensitivity and specificity, the Human S100A8 ELISA Kit offers accurate and reproducible results, making it an essential tool for researchers exploring the complex interplay between inflammation and disease. This kit is a valuable asset for cutting-edge research in the fields of immunology, inflammation, and biomarker discovery.
Product Name:
Human S100A8 ELISA Kit
SKU:
HUFI01619
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.375 ng/mL
Range:
0.625-40 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
92-102%
88-105%
85-102%
EDTA Plasma (n = 5)
82-98%
82-89%
85-95%
Heparin Plasma (n = 5)
80-98%
81-100%
82-95%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-97
93
EDTA Plasma (n = 5)
88-104
96
Heparin Plasma (n = 5)
85-105
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
