Human SC5b9 (soluble terminal complement complex) ELISA Kit
The Human SC5b-9 (Soluble Terminal Complement Complex) ELISA Kit is specifically designed for the precise detection of soluble SC5b-9 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and replicable results, making it a valuable tool for various research applications.The soluble terminal complement complex SC5b-9 is a key component of the complement system, playing a critical role in immune response and inflammation. Abnormal levels of SC5b-9 have been associated with various autoimmune diseases, infections, and inflammatory conditions, making it a vital biomarker for understanding these diseases and developing potential treatments. With the Human SC5b-9 ELISA Kit, researchers can accurately measure SC5b-9 levels in biological samples, advancing our understanding of the complement system and its role in health and disease. This kit is an essential resource for scientists seeking to explore the mechanisms of immune response and discover new therapeutic targets.
Product Name:
Human SC5b9 (soluble terminal complement complex) ELISA Kit
SKU:
HUES03247
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 ng/mL
Detection range:
31.25-2000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
