Description
| Product Name: | Human SH2D1B Recombinant Protein |
| Product Code: | RPPB4671 |
| Size: | 10µg |
| Species: | Human |
| Target: | SH2D1B |
| Synonyms: | SH2 Domain Containing 1B, EWS/FLI1-Activated Transcript 2, EAT-2, EAT2, SH2 Domain-Containing Molecule EAT2, SH2 Domain-Containing Protein 1B, SH2 domain-containing protein 1B, EWS/FLI1-activated transcript 2, EAT-2. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | SH2D1B protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPMDLPYYH GRLTKQDCET LLLKEGVDGN FLLRDSESIP GVLCLCVSFK NIVYTYRIFR EKHGYYRIQT AEGSPKQVFP SLKELISKFE KPNQGMVVHL LKPIKRTSPS LRWRGLKLEL ETFVNSNSDY VDVLPHHHHH H |
SH2 domain-containing protein 1B, also known as SH2D1B activates NK cells. SH2D1B participates in controlling signal transduction through at least 4 receptors, CD84, SLAMF1, LY9 and CD244, expressed on the surface of professional antigen-presenting cells. Two isoforms of the human SH2D1B are produced by alternative splicing. Superior Mesenteric Artery Syndrome is one of the diseases which is associated with SH2D1B.�
SH2D1B Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 141 amino acids (1-132a.a.) and having a molecular mass of 16.4kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). SH2D1B is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244 (PubMed:11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation; downstream signaling involves PLCG1 and ERK activation (PubMed:24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling. In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse (PubMed:24687958). Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation. |
| NCBI Summary: | By binding phosphotyrosines through its free SRC (MIM 190090) homology-2 (SH2) domain, EAT2 regulates signal transduction through receptors expressed on the surface of antigen-presenting cells (Morra et al., 2001 [PubMed 11689425]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | O14796 |
| NCBI GenInfo Identifier: | 23503051 |
| NCBI Gene ID: | 117157 |
| NCBI Accession: | O14796.2 |
| UniProt Secondary Accession: | O14796,Q5T0L1, Q8NI18, Q969K9, B2RBN6, |
| UniProt Related Accession: | O14796 |
| Molecular Weight: | 9,024 Da |
| NCBI Full Name: | SH2 domain-containing protein 1B |
| NCBI Synonym Full Names: | SH2 domain containing 1B |
| NCBI Official Symbol: | SH2D1B�� |
| NCBI Official Synonym Symbols: | EAT2�� |
| NCBI Protein Information: | SH2 domain-containing protein 1B |
| UniProt Protein Name: | SH2 domain-containing protein 1B |
| UniProt Synonym Protein Names: | EWS/FLI1-activated transcript 2; EAT-2 |
| Protein Family: | SH2 domain-containing protein |
| UniProt Gene Name: | SH2D1B�� |
