The Human sST2 (Soluble ST2) ELISA Kit is specifically developed to quantitatively detect soluble ST2 protein levels in diverse human biological samples. Soluble ST2, a member of the interleukin-1 receptor family, is a crucial biomarker associated with inflammatory responses, allergic reactions, cardiovascular diseases, and other pathological conditions. Accurate measurement of soluble ST2 is imperative for understanding its role in immune regulation and inflammatory processes and for evaluating disease severity and prognosis accurately. Our ELISA kit is meticulously designed to deliver exceptional sensitivity and specificity, ensuring precise and reproducible outcomes in the measurement of soluble ST2 levels. Manufactured under rigorous quality control standards, this kit offers robust performance, making it an ideal choice for both research studies and clinical applications. Count on the Human sST2 ELISA Kit from Assay Genie for reliable and accurate quantification of this essential biomarker in your scientific inquiries.
Product Name:
Human sST2 (Soluble ST2) ELISA Kit
SKU:
AEES00118
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
