Human SULT1E1 Recombinant Protein (RPPB5827)
- SKU:
- RPPB5827
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P49888
- Research Area:
- Enzymes
Description
| Product Name: | Human SULT1E1 Recombinant Protein |
| Product Code: | RPPB5827 |
| Size: | 25µg |
| Species: | Human |
| Target: | SULT1E1 |
| Synonyms: | EST, STE, EST-1, MGC34459, SULT1E1, Estrogen sulfotransferase, Sulfotransferase estrogen-preferring. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The SULT1E1 protein solution contains 20mM Tris-HCl, pH-8 and 20% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MNSELDYYEK FEEVHGILMY KDFVKYWDNV EAFQARPDDL VIATYPKSGT TWVSEIVYMI YKEGDVEKCK EDVIFNRIPF LECRKENLMN GVKQLDEMNS PRIVKTHLPP ELLPASFWEK DCKIIYLCRN AKDVAVSFYY FFLMVAGHPN PGSLPEFVEK FMQGQVPYGS WYKHVKSWWE KGKSPRVLFL FYEDLKEDIR KEVIKLIHFL ERKPSEELVD RIIHHTSFQE MKNNPSTNYT TLPDEIMNQK LSPFMRKGIT GDWKNHFTVA LNEKFDKHYE QQMKESTLKF RTEILEHHHH HH |
SULT1E1 catalyzes the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. Decreased SULT1E1 expression is linked with estrogen-dependent endometrial carcinomas. Altered cellular proliferation was detected in cells stably expressing SULT1E1.
Recombinant Human SULT1E1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 302 amino acids (1-294 a.a.) and having a molecular mass of 36.1 kDa. SULT1E1 is fused to 6 amino acid His Tag at C-terminus and purified by conventional chromatography techniques.
| UniProt Protein Function: | SULT1E1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated. Belongs to the sulfotransferase 1 family. |
| UniProt Protein Details: | Protein type:EC 2.8.2.4; Transferase; Energy Metabolism - sulfur; Lipid Metabolism - androgen and estrogen Chromosomal Location of Human Ortholog: 4q13.1 Cellular Component: cytosol Molecular Function:steroid sulfotransferase activity; estrone sulfotransferase activity; flavonol 3-sulfotransferase activity; steroid binding Biological Process: steroid metabolic process; estrogen metabolic process; xenobiotic metabolic process; sulfation; female pregnancy; 3'-phosphoadenosine 5'-phosphosulfate metabolic process |
| NCBI Summary: | Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49888 |
| NCBI GenInfo Identifier: | 1711604 |
| NCBI Gene ID: | 6783 |
| NCBI Accession: | P49888.1 |
| UniProt Related Accession: | P49888 |
| Molecular Weight: | 35kDa |
| NCBI Full Name: | Estrogen sulfotransferase |
| NCBI Synonym Full Names: | sulfotransferase family 1E member 1 |
| NCBI Official Symbol: | SULT1E1�� |
| NCBI Official Synonym Symbols: | EST; STE; EST-1; ST1E1�� |
| NCBI Protein Information: | estrogen sulfotransferase |
| UniProt Protein Name: | Estrogen sulfotransferase |
| UniProt Synonym Protein Names: | EST-1; Sulfotransferase 1E1; ST1E1; Sulfotransferase, estrogen-preferring |
| Protein Family: | Estrogen sulfotransferase |
| UniProt Gene Name: | SULT1E1�� |
| UniProt Entry Name: | ST1E1_HUMAN |
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