Human TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit
The Human TCC (C5b-9 Terminal Complement Complex) ELISA Kit is specifically designed for the precise measurement of TCC levels in human biological samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for various research applications. The TCC, also known as the C5b-9 complex, is a key component of the complement system involved in immune response and inflammation. Dysregulation of the complement system has been implicated in various diseases, including autoimmune disorders, infections, and inflammatory conditions.
The TCC ELISA kit enables researchers to study the role of this complex in disease pathogenesis and explore its potential as a therapeutic target. Overall, the Human TCC ELISA Kit offers a valuable tool for investigating the function of the complement system and its implications in health and disease. Its reliability and precision make it an essential asset for researchers in the field of immunology and inflammation.
Product Name:
Human TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit
SKU:
AEFI00357
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
7.5 ng/mL
Range:
12.5-800 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-103%
82-99%
82-97%
EDTA Plasma (n = 5)
85-101%
84-100%
86-100%
Heparin Plasma (n = 5)
87-105%
90-99%
92-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-99
93
EDTA Plasma (n = 5)
85-102
96
Heparin Plasma (n = 5)
88-104
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
