Description
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Human TGF beta Array (25 targets) (SARB0070)
The Human TGF-beta Array (SARB0070) offered by Assay Genie is a powerful tool for researchers studying the transforming growth factor beta (TGF-beta) pathway. This array allows for the simultaneous detection of 25 TGF-beta related targets, providing a comprehensive view of the signaling network involved in cell growth, differentiation, and immune response.With high sensitivity and specificity, this array is ideal for analyzing TGF-beta signaling in various cell types and conditions. Researchers can easily track changes in protein expression levels and phosphorylation status, making it an invaluable tool for studies on cancer, fibrosis, and immune regulation.
Validated for use in ELISA and other protein detection assays, the Human TGF-beta Array (SARB0070) offers a cost-effective and time-saving solution for investigating the intricate mechanisms of TGF-beta signaling. Gain deeper insights into the role of TGF-beta in health and disease with this comprehensive array from Assay Genie.
| Product Sku: | SARB0070 |
| Size: | 2, 4, or 8 |
| Species Detected: | Human |
| Number of Targets Detected: | 25 |
| Gene Symbols: | ACVR1, BAMBI, BMP15, BMP2, BMP4, BMP6, BMP7, BMP8A, BMPR1A, BMPR2|FST, GDF11, GDF15, GDF2, INHBA, LEFTY2, MADH3, NOG, SMAD4, TGFA, TGFB1, TGFB2, TGFBI, TGFBR2, TGFBR3 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
Activin A
| Activin R1A (ALK-2)
| BAMBI (NMA)
| beta IG-H3
| BMP-2
|
BMP-4
| BMP-6
| BMP-7
| BMP-8
| BMP-9
|
BMP-15 (GDF-9B)
| BMPR-IA (ALK-3)
| BMPR-II
| Follistatin
| GDF-11 (BMP-11)
|
GDF-15
| Lefty-A
| Noggin
| SMAD3
| SMAD4
|
TGF alpha
| TGF beta 1
| TGF beta 2
| TGF-beta RII
| TGF beta R3
|
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Human TGF beta Array C2 Membranes
- Blocking Buffer
- Biotinylated Antibody Cocktail
- 1,000X HRP-Streptavidin Concentrate
- 20X Wash Buffer I Concentrate
- 20X Wash Buffer II Concentrate
- 2X Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- Plastic Sheets
- Array Map Template
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated anti-IgG |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
