The Human TLN (Talin) ELISA Kit is meticulously designed to quantitatively detect and measure levels of Talin in various human biological samples. Talin, a vital cytoskeletal protein, plays a crucial role in cell adhesion and interactions with the extracellular matrix, serving as a key regulator of cellular processes like migration, growth, and differentiation. Accurate measurement of Talin with our ELISA kit provides valuable insights into cell adhesion dynamics, cytoskeletal organization, and cellular signaling pathways. This kit ensures exceptional sensitivity and specificity, guaranteeing reliable and reproducible results. Engineered under strict quality control measures, it delivers robust performance and user-friendly operation, making it an optimal choice for both research and clinical applications. Rely on the Human TLN ELISA Kit for precise and dependable quantification of this essential biomarker in your studies.
Product Name:
Human TLN (Talin) ELISA Kit
SKU:
AEES00108
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
