Human TNFSF11/RANKL (Tumor necrosis factor ligand superfamily member 11) ELISA Kit
The Human TNFSF11 (RANKL) ELISA Kit is specifically designed for the precise measurement of tumor necrosis factor ligand superfamily member 11 levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and accuracy, this kit ensures dependable and consistent results, making it an indispensable tool for various research applications. TNFSF11, also known as RANKL, is a key regulator of bone remodeling and immune response, playing a critical role in various physiological processes including osteoclast differentiation and activation.
Dysregulation of TNFSF11 has been associated with various diseases such as osteoporosis, arthritis, and cancer, highlighting its importance as a potential therapeutic target and diagnostic biomarker. Overall, the Human TNFSF11 (RANKL) ELISA Kit provides researchers with a reliable and effective means of quantifying TNFSF11 levels in human samples, aiding in the understanding of its biological functions and implications in disease pathogenesis.
Product Name:
Human TNFSF11/RANKL (Tumor necrosis factor ligand superfamily member 11) ELISA Kit
SKU:
AEFI00313
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-104%
83-99%
82-99%
EDTA Plasma (n = 5)
91-105%
84-101%
88-100%
Heparin Plasma (n = 5)
87-99%
82-101%
85-97%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-101
93
EDTA Plasma (n = 5)
93-105
98
Heparin Plasma (n = 5)
87-104
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
