The Human TRAIL-R1 (TNFRSF10A) ELISA Kit is a cutting-edge assay designed for the precise measurement of TNFRSF10A levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for a variety of research applications. TNFRSF10A, also known as TRAIL-R1, is a member of the tumor necrosis factor receptor superfamily, playing a crucial role in the regulation of apoptosis and immune responses. Dysregulation of TNFRSF10A has been linked to various diseases including cancer, autoimmune disorders, and inflammatory conditions, highlighting its importance as a potential therapeutic target and biomarker for disease progression.
By using the Human TRAIL-R1 (TNFRSF10A) ELISA Kit, researchers can gain valuable insights into the role of TNFRSF10A in disease pathogenesis and explore its potential as a target for novel treatment strategies. Trust in the accuracy and reliability of this ELISA kit for robust and reproducible results in your studies.
Product Name:
Human TRAIL R1/TNFRSF10A ELISA Kit
SKU:
HUFI00494
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1
2
3
Serum (n = 5)
86-100%
83-100%
82-98%
EDTA Plasma (n = 5)
90-102%
86-99%
81-91%
Heparin Plasma (n = 5)
86-101%
89-96%
80-92%
Recovery:
Sample
1
2
Serum (n = 5)
88-105
99
EDTA Plasma (n = 5)
91-104
96
Heparin Plasma (n = 5)
85-101
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
