Human TRDMT1 Recombinant Protein (RPPB2349)
- SKU:
- RPPB2349
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O14717
- Research Area:
- Enzymes
Description
| Product Name: | Human TRDMT1 Recombinant Protein |
| Product Code: | RPPB2349 |
| Size: | 10µg |
| Species: | Human |
| Target: | TRDMT1 |
| Synonyms: | tRNA (cytosine(38)-C(5))-methyltransferase, DNA (cytosine-5)-methyltransferase-like protein 2, Dnmt2, DNA methyltransferase homolog HsaIIP, DNA MTase homolog HsaIIP, M.HsaIIP, PuMet, TRDMT1, DNMT2, DMNT2, RNMT1, MHSAIIP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The TRDMT1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMEPLRV LELYSGVGGM HHALRESCIP AQVVAAIDVN TVANEVYKYN FPHTQLLAKT IEGITLEEFD RLSFDMILMS PPCQPFTRIG RQGDMTDSRT NSFLYILDIL PRLQKLPKYI LLENVKGFEV SSTRDLLIQT IENCGFQYQE FLLSPTSLGI PNSRLRYFLI AKLQSEPLPF QAPGQVLMEF PKIESVHPQK YAMDVENKIQ EKNVEPNISF DGSIQCSGKD AILFKLETAE EIHRKNQQDS DLSVKMLKDF LEDDTDVNQY LLPPKSLLRY ALLLDIVQPT CRRSVCFTKG YGSYIEGTGS VLQTAEDVQV ENIYKSLTNL SQEEQITKLL ILKLRYFTPK EIANLLGFPP EFGFPEKITV KQRYRLLGNS LNVHVVAKLI KILYE |
tRNA aspartic acid methyltransferase 1 (TRDMT1) is a member of the C5-methyltransferase family. TRDMT1 specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). TRDMT1 is a protein responsible for the methylation of aspartic acid transfer RNA, particularly at the cytosine-38 residue in the anticodon loop. In addition, the TRDMT1 enzyme has residual DNA-(cytosine-C5) methyltransferase activity. TRDMT1 is expressed ubiquitously; however it has a higher expression in the testis, ovary and thymus and at much lower levels in the spleen, prostate, colon, small intestine, and peripheral blood leukocytes. Though similar in sequence and structure to DNA cytosine methyltransferases, the TRDMT1 gene is distinctive and extremely conserved in its function between taxa.
TRDMT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 415 amino acids (1-391) and having a molecular mass of 47.2kDa.TRDMT1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TRDMT1: Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). Belongs to the C5-methyltransferase family. 6 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.204; Methyltransferase; Methyltransferase, tRNA Chromosomal Location of Human Ortholog: 10p13 Cellular Component: cytoplasm; nucleoplasm Molecular Function:tRNA (cytosine-5-)-methyltransferase activity; tRNA methyltransferase activity Biological Process: tRNA methylation; tRNA modification |
| NCBI Summary: | This gene encodes a protein responsible for the methylation of aspartic acid transfer RNA, specifically at the cytosine-38 residue in the anticodon loop. This enzyme also possesses residual DNA-(cytosine-C5) methyltransferase activity. While similar in sequence and structure to DNA cytosine methyltransferases, this gene is distinct and highly conserved in its function among taxa. [provided by RefSeq, Jun 2010] |
| UniProt Code: | O14717 |
| NCBI GenInfo Identifier: | 17374834 |
| NCBI Gene ID: | 1787 |
| NCBI Accession: | O14717.1 |
| UniProt Secondary Accession: | O14717,O43669, Q86WW6, B0YJ02, B0YJ03, B0YJ07, B0YJ08 |
| UniProt Related Accession: | O14717 |
| Molecular Weight: | 45kDa |
| NCBI Full Name: | tRNA (cytosine(38)-C(5))-methyltransferase |
| NCBI Synonym Full Names: | tRNA aspartic acid methyltransferase 1 |
| NCBI Official Symbol: | TRDMT1�� |
| NCBI Official Synonym Symbols: | DMNT2; DNMT2; PUMET; RNMT1; MHSAIIP�� |
| NCBI Protein Information: | tRNA (cytosine(38)-C(5))-methyltransferase |
| UniProt Protein Name: | tRNA (cytosine(38)-C(5))-methyltransferase |
| UniProt Synonym Protein Names: | DNA (cytosine-5)-methyltransferase-like protein 2; Dnmt2; DNA methyltransferase homolog HsaIIP; DNA MTase homolog HsaIIP; M.HsaIIP; PuMet |
| Protein Family: | tRNA |
| UniProt Gene Name: | TRDMT1�� |
