Human TSLP Recombinant Protein (RPPB1044)
- SKU:
- RPPB1044
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q969D9
- Research Area:
- Cytokines
Description
| Product Name: | Human TSLP Recombinant Protein |
| Product Code: | RPPB1044 |
| Size: | 10µg |
| Species: | Human |
| Target: | TSLP |
| Synonyms: | Thymic Stromal Lymphopoietin, TSLP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a concentrated solution (1mg/ml) contains 130mM NaCl, and 20mM sodium phosphate, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized TSLP in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized TSLP although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TSLP should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ |
| Biological Activity: | The ED50 is determined by the ability to induce proliferation of human Interleukin-7 receptor alpha & human TSLP Receptor co-transfected with mouse BaF3 cells which was found to be less than 0.3ng/ml. |
TSLP protein is a hemopoietic cytokine which signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor & the Interleukin-7 receptor alpha chain. TSLP impacts myeloid cells thus induces the discharge of T cell-attracting chemokines from monocytes & increases the growth of CD11c(+) dendritic cells. TSLP is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the Interleukin-7 receptor alpha chain & the TSLPR. Similar to IL-7, TSLP enhances phosphorylation of STAT3 and STAT5, though uses kinases excluding JAKs for its activation. TSLP induces the release of T cell-attracting chemokines such asTARC & MDC from monocytes & triggers CD11c(+) dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as Iinterleukin-4, Interleukin-5, Interleukin-13 and TNF-alpha whereas down-regulating Interleukin-10 and IFN-gamma play a role in the initiation of allergic inflammation.
TSLP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15 kDa.The TSLP is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TSLP: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 5q22.1 Cellular Component: extracellular space Molecular Function:cytokine activity |
| NCBI Summary: | This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| UniProt Code: | Q969D9 |
| NCBI GenInfo Identifier: | 74751719 |
| NCBI Gene ID: | 85480 |
| NCBI Accession: | Q969D9.1 |
| UniProt Secondary Accession: | Q969D9,Q8IW99, Q96AU7, G3XAM8, |
| UniProt Related Accession: | Q969D9 |
| Molecular Weight: | 7,426 Da |
| NCBI Full Name: | Thymic stromal lymphopoietin |
| NCBI Synonym Full Names: | thymic stromal lymphopoietin |
| NCBI Official Symbol: | TSLP�� |
| NCBI Protein Information: | thymic stromal lymphopoietin |
| UniProt Protein Name: | Thymic stromal lymphopoietin |
| Protein Family: | Thymic stromal lymphopoietin |
| UniProt Gene Name: | TSLP�� |
| UniProt Entry Name: | TSLP_HUMAN |
