Human UFD1L Recombinant Protein (RPPB5078)
- SKU:
- RPPB5078
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q92890
Description
| Product Name: | Human UFD1L Recombinant Protein |
| Product Code: | RPPB5078 |
| Size: | 10µg |
| Species: | Human |
| Target: | UFD1L |
| Synonyms: | Ubiquitin Fusion Degradation 1 Like (Yeast), UB Fusion Protein 1, Ubiquitin Fusion Degradation Protein 1 Homolog, UFD1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The UFD1L solution contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 1mM DTT and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMFSFNMF DHPIPRVFQN RFSTQYRCFS VSMLAGPNDR SDVEKGGKII MPPSALDQLS RLNITYPMLF KLTNKNSDRM THCGVLEFVA DEGICYLPHW MMQNLLLEEG GLVQVESVNL QVATYSKFQP QSPDFLDITN PKAVLENALR NFACLTTGDV IAINYNEKIY ELRVMETKPD KAVSIIECDM NVDFDAPLGY KEPERQVQHE ESTEGEADHS GYAGELGFRA FSGSGNRLDG KKKGVEPSPS PIKPGDIKRG IPNYEFKLGK ITFIRNSRPL VKKVEEDEAG GRFVAFSGEG QSLRKKGRKP |
UFD1L forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.
UFD1L Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 330 amino acids (1-307) and having a molecular mass of 36.9kDa.UFD1L is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UFD1L: Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures. Belongs to the UFD1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin-specific protease; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: cytosol; nucleus Molecular Function:protein binding; ubiquitin-specific protease activity; protein complex binding; receptor binding Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; skeletal development |
| NCBI Summary: | The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Jun 2009] |
| UniProt Code: | Q92890 |
| NCBI GenInfo Identifier: | 160332310 |
| NCBI Gene ID: | 7353 |
| NCBI Accession: | Q92890.3 |
| UniProt Secondary Accession: | Q92890,Q9Y5N0, A8MW31, |
| UniProt Related Accession: | Q92890 |
| Molecular Weight: | 307 |
| NCBI Full Name: | Ubiquitin fusion degradation protein 1 homolog |
| NCBI Synonym Full Names: | ubiquitin fusion degradation 1 like (yeast) |
| NCBI Official Symbol: | UFD1L�� |
| NCBI Official Synonym Symbols: | UFD1�� |
| NCBI Protein Information: | ubiquitin fusion degradation protein 1 homolog; UB fusion protein 1 |
| UniProt Protein Name: | Ubiquitin fusion degradation protein 1 homolog |
| UniProt Gene Name: | UFD1L�� |
| UniProt Entry Name: | UFD1_HUMAN |
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