Human Virus Induced Signaling Adapter (VISA) ELISA Kit
The Human Virus-Induced Signaling Adapter (VISA) ELISA Kit is a cutting-edge tool for the precise measurement of VISA levels in human samples such as serum, plasma, and cell culture supernatants. This advanced kit offers unparalleled sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.VISA is a key adapter molecule involved in the innate immune response to viral infections, playing a crucial role in signaling pathways that trigger the production of antiviral cytokines.
Understanding the regulation of VISA is essential for studying viral pathogenesis and developing novel strategies for combating viral diseases.With the Human VISA ELISA Kit, researchers can confidently investigate the role of VISA in host defense mechanisms, immune responses, and viral pathogenicity. This kit provides a valuable tool for unlocking new insights into viral infection and immunity, paving the way for innovative therapeutic interventions.
Product Name:
Human Virus Induced Signaling Adapter (VISA) ELISA Kit
Product Code:
HUDL03098
Size:
96 Assays
Target:
VISA
Synonyms:
CARDIF, IPS1, MAVS, Mitochondrial Antiviral-Signaling Protein, Interferon beta promoter stimulator protein 1, CARD Adaptor Inducing IFN-beta, NF-kappa-B-activating protein 031N
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
