Human Von Willebrand Factor Cleaving Protease (vWFCP) ELISA Kit
The Human Von Willebrand Factor Cleaving Protease (VWFCP) ELISA Kit is a powerful tool for the accurate measurement of VWFCP levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing reliable and consistent results that are essential for various research applications.VWFCP is an important protease involved in the regulation of von Willebrand Factor (VWF) levels and function in the blood coagulation cascade. Dysregulation of VWFCP activity has been implicated in various bleeding disorders, making it a critical biomarker for studying these conditions and developing potential therapeutic interventions.
By utilizing the Human VWFCP ELISA Kit, researchers can gain valuable insights into the role of VWFCP in hemostasis and thrombosis, as well as its potential as a target for novel therapeutic strategies. With its user-friendly protocol and robust performance, this kit is an indispensable tool for advancing our understanding of VWFCP biology and its implications for human health.
Product Name:
Human Von Willebrand Factor Cleaving Protease (vWFCP) ELISA Kit
Product Code:
HUDL03113
Size:
96 Assays
Target:
vWFCP
Synonyms:
ADAMTS13, TTP, vWF-cp, vWF-cleaving protease, A Disintegrin And Metalloproteinase With A Thrombospondin Type 1 Motif Member 13
Detection Method:
Sandwich
Range:
62.5-4,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
