Human VWFCP/ADAMTS13 (Von Willebrand Factor Cleaving Protease) ELISA Kit
The Human VWF:CP (ADAMTS13-von Willebrand Factor Cleaving Protease) ELISA Kit is specifically designed for the accurate detection of VWF:CP levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an excellent tool for various research applications.VWF:CP, also known as ADAMTS13, is a critical enzyme involved in regulating von Willebrand factor (VWF) activity by cleaving it and preventing excessive blood clotting. Dysregulation of this enzyme has been linked to various blood disorders, such as thrombotic thrombocytopenic purpura (TTP), making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.The Human VWF:CP ELISA Kit from Assay Genie offers researchers a highly reliable and efficient method for quantifying VWF:CP levels in human samples, enabling accurate and insightful investigations into the role of this enzyme in health and disease.
Product Name:
Human VWFCP/ADAMTS13 (Von Willebrand Factor Cleaving Protease) ELISA Kit
SKU:
HUES02566
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 2 vials, 20 mL | 96T*5: 10 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
99-114
90-101
98-114
Average (%)
105
95
104
1:4
Range (%)
95-109
79-90
88-100
Average (%)
100
85
93
1:8
Range (%)
91-103
81-93
83-96
Average (%)
98
87
88
1:16
Range (%)
89-102
81-91
82-95
Average (%)
94
86
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
93-106
100
Cell Culture Media (n=8)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.01
2.88
7.72
1.02
3.03
8.42
Standard deviation
0.07
0.14
0.33
0.06
0.2
0.64
C V (%)
6.97
4.7
4.3
6.05
6.74
7.55
Uniport ID:
Q76LX8
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human VWFCP/ADAMTS13 in samples. No significant cross-reactivity or interference between Human VWFCP/ADAMTS13 and analogues was observed
