The IL10 Antibody (CAB2171) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has high reactivity with human samples, making it suitable for use in a variety of research applications including Western blotting.Interleukin-10 is known for its role in dampening immune responses and promoting immune tolerance, making it a critical mediator in various disease states such as infections, autoimmunity, and inflammation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
IL10 Antibody
SKU:
CAB2171
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CSIF, TGIF, GVHDS, IL-10, IL10A, IL10
Positive Sample:
RAW264.7 treated with LPS and Brefeldin A
Cellular Localization:
Secreted.
Calculated MW:
21kDa
Observed MW:
18kDa
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Purification Method
Affinity purification
Gene ID
3586
RRID
AB_2764189
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using IL10 Rabbit pAb (CAB2171) at 1:800 dilution. Raw264. 7 cells were treated with LPS for 6 hours and Brefeldin A for 3 hours of stimulation. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using IL10 Rabbit pAb (CAB2171) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
![APC Anti-Human IL-10 Antibody [JES3-9D7] (AGEL2034)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/22565/607212/apc-anti-human-il-10-antibody-jes3-9d7-agel2034__96634.1707500461.jpg?c=2 "APC Anti-Human IL-10 Antibody [JES3-9D7] (AGEL2034)")
![APC Anti-Mouse IL-10 Antibody [JES5-16E3] (AGEL2029)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/22560/606412/apc-anti-mouse-il-10-antibody-jes5-16e3-agel2029__92701.1707497936.jpg?c=2 "APC Anti-Mouse IL-10 Antibody [JES5-16E3] (AGEL2029)")
