IRX4 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IF, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ug format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
IRX4 Antibody
SKU:
PACO54314
Size:
50μg
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant Human Iroquois-class homeodomain protein IRX-4 protein (287-363AA)
Immunogen Species:
Homo sapiens (Human)
Uniprot No:
P78413
Form:
Liquid
Tested Applications:
ELISAWBIF
Recommended Dilution:
Application
Recommended Dilution
WB
1:500-1:2000
IF
1:50-1:200
Synonyms:
Homeodomain protein IRXA3 antibody, Iroquois homeobox protein 4 antibody, Iroquois-class homeodomain protein IRX-4 antibody, Iroquoisclass homeodomain protein IRX4 antibody, Irx4 antibody, IRX4_HUMAN antibody, IRXA3 antibody
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, Colo320 whole cell lysate All lanes: IRX4 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55, 58 kDa Observed band size: 55 kDa
Immunofluorescence staining of MCF-7 cells with PACO54314 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
