The KDM4A Polyclonal Antibody (CAB7953) is a valuable tool for researchers studying KDM4A, a key epigenetic regulator involved in histone demethylation. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards KDM4A in human samples, making it an ideal choice for Western blot applications. By binding to the KDM4A protein, this antibody enables the accurate detection and analysis of KDM4A expression in a variety of cell types.KDM4A plays a crucial role in gene regulation and chromatin remodeling, impacting processes such as cell differentiation, development, and disease progression.
Its dysregulation has been implicated in various cancers, making it a promising target for therapeutic interventions. By studying the function of KDM4A using this antibody, researchers can gain valuable insights into the mechanisms underlying cancer and potentially identify novel treatment strategies targeting KDM4A-dependent pathways.
Product Name:
KDM4A Rabbit Polyclonal Antibody
SKU:
CAB7953
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 585-815 of human KDM4A (NP_055478.2).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
JMJD2; JHDM3A; JMJD2A; TDRD14A; KDM4A
Positive Sample:
BT-474,THP-1,Mouse thymus
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
121kDa
Observed MW:
150kDa
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
Purification Method:
Affinity purification
Gene ID:
9682
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using KDM4A Rabbit pAb (CAB7953) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
