The KDM4A Monoclonal Antibody (CAB9267) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it an ideal choice for immunohistochemistry and immunofluorescence applications.KDM4A, also known as Jumonji domain-containing protein 2A, plays a crucial role in histone demethylation and transcriptional regulation. Dysregulation of KDM4A has been linked to various diseases, including cancer, neurodevelopmental disorders, and metabolic diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
KDM4A Monoclonal Antibody
SKU:
CAB9267
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1503
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
GDGR VTVG EPCT RKKG SAAR SFSE RELA EVAD EYMF SLEE NKKS KGRR QPLS KLPR HHPL VLQE CVSD DETS EQLT PEEE AEET EAWA KPLS QLWQ NRPP N
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
JMJD2, JHDM3A, JMJD2A, TDRD14A, KDM4A
Positive Sample:
HeLa, NIH/3T3, SH-SY5Y
Cellular Localization:
Nucleus.
Calculated MW:
121kDa
Observed MW:
150kDa
This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.
Purification Method
Affinity purification
Gene ID
9682
RRID
AB_2863701
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using KDM4A Rabbit mAb (CAB9267) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
