The [KO Validated] KDM5B Antibody (CAB7772) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high specificity and sensitivity in detecting KDM5B in human samples, and has been validated for use in Western blot applications.KDM5B, also known as JARID1B, is a member of the Jumonji family of histone demethylases and plays a critical role in regulating gene expression by removing methyl groups from histone proteins. Dysregulation of KDM5B has been linked to various diseases, including cancer, neurodevelopmental disorders, and metabolic syndromes, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] KDM5B Antibody
SKU:
CAB7772
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10765
RRID
AB_2770071
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and KDM5B knockout (KO) 293T cells, using [KO Validated] KDM5B Rabbit pAb (CAB7772) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of L929 cells using [KO Validated] KDM5B Rabbit pAb (CAB7772) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
