The KHDRBS1/Sam68 Antibody (CAB6101) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in a variety of applications, with a particular emphasis on immunohistochemistry and western blotting.KHDBRS1, also known as Sam68, is a multifunctional RNA-binding protein that plays a critical role in alternative splicing and gene expression. Its dysregulation has been implicated in various diseases, including cancer and neurological disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
KHDRBS1/Sam68 Antibody
SKU:
CAB6101
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p62, p68, Sam68, KHDRBS1/Sam68
Positive Sample:
SKOV3, HeLa, Jurkat, PC-12, Mouse brain, Rat brain
Cellular Localization:
Membrane, Nucleus.
Calculated MW:
48kDa
Observed MW:
68kDa
This gene encodes a member of the K homology domain-containing, RNA-binding, signal transduction-associated protein family. The encoded protein appears to have many functions and may be involved in a variety of cellular processes, including alternative splicing, cell cycle regulation, RNA 3'-end formation, tumorigenesis, and regulation of human immunodeficiency virus gene expression. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10657
RRID
AB_2766746
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using KHDRBS1/Sam68 Rabbit pAb (CAB6101) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human prostate cancer using KHDRBS1/Sam68 Rabbit pAb (CAB6101) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human prostate cancer using KHDRBS1/Sam68 Rabbit pAb (CAB6101) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human stomach using KHDRBS1/Sam68 Rabbit pAb (CAB6101) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Confocal immunofluorescence analysis of HeLa cells using KHDRBS1/Sam68 Rabbit pAb (CAB6101) at dilution of 1:400. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using KHDRBS1/Sam68 Rabbit pAb (CAB6101) at dilution of 1:400. Blue: DAPI for nuclear staining.
