The [KO Validated] c-Myc Monoclonal Antibody (CAB19032) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, exhibits strong reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.The c-Myc protein is a transcription factor with important roles in various cellular processes, including cell cycle progression and metabolism. Dysregulation of c-Myc expression is implicated in numerous diseases, particularly cancer, making it a prominent target for therapeutic intervention.
This antibody is validated for use in WB, ChIP, ELISA, CUT&Tag applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] c-Myc Monoclonal Antibody
SKU:
CAB19032
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0412
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP
5μg antibody for 10μg-15μg of Chromatin
CUT&Tag
10⁵ cells /1 μg
Synonyms:
MRTL, MYCC, c-Myc, bHLHe39, yc
Positive Sample:
Jurkat, MCF7, HCT 116
Cellular Localization:
Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:
51kDa
Observed MW:
50-60kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method
Affinity purification
Gene ID
4609
RRID
AB_2862524
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using [KO Validated] c-Myc Rabbit mAb (CAB19032) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from wild type (WT) and c-Myc knockout (KO) HCT 116 cells, using [KO Validated] c-Myc Rabbit mAb (CAB19032) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
UT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10⁵ K562 cells with 1μg c-Myc Rabbit mAb(CAB19032), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of ESE1 in representative gene loci (NPM1), as shown in figure.
