The CAPN1 Polyclonal Antibody (CAB18006) is a valuable tool for researchers studying CAPN1, a calpain family member implicated in various cellular processes such as signal transduction, apoptosis, and cell migration. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to CAPN1, the antibody allows for the detection and analysis of this protein in a variety of cell types, making it an ideal choice for studies in molecular biology and cell signaling pathways.
CAPN1, a calcium-dependent protease, plays a crucial role in regulating protein turnover and cell function. Dysregulation of CAPN1 activity has been associated with diseases such as cancers, neurodegenerative disorders, and muscular dystrophies, highlighting its importance as a potential therapeutic target. By further understanding the functions of CAPN1, researchers can gain insights into disease mechanisms and potentially identify new treatment strategies.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
823
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Calpain 1 knockout (KO) HeLa cells, using [KO Validated] Calpain 1 Rabbit pAb (CAB18006) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
