The [KO Validated] Cyclin B1 Monoclonal Antibody (CAB19037) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, has been validated for knockout (KO) samples and is highly reactive with human samples.Cyclin B1 is a crucial regulator of cell division and plays a pivotal role in cell cycle progression. Dysregulation of Cyclin B1 expression has been linked to various human diseases, including cancer. The KO Validated Cyclin B1 Rabbit Monoclonal Antibody (CAB19037) binds specifically to Cyclin B1, enabling accurate detection and analysis in a variety of cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
[KO Validated] Cyclin B1 Monoclonal Antibody
SKU:
CAB19037
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0474
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.
Purification Method
Affinity purification
Gene ID
891
RRID
AB_2862529
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and Cyclin B1 knockout (KO) HeLa cells using [KO Validated] Cyclin B1 Rabbit mAb (CAB19037) at 1:5000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Western blot analysis of lysates from Jurkat cells using [KO Validated] Cyclin B1 Rabbit mAb (CAB19037) at 1:5000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
