The [KO Validated] Galectin 3/LGALS3 Antibody (CAB13506) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. LGALS3, also known as Gal-3, is a member of the galectin family of proteins and plays a crucial role in inflammation, fibrosis, and cancer progression. Its involvement in these processes makes it a valuable target for research in immunology and oncology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
[KO Validated] Galectin 3/LGALS3 Antibody
SKU:
CAB13506
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
L31, GAL3, MAC2, CBP35, GALBP, GALIG, LGALS2, S3
Positive Sample:
HeLa, U-87MG, C2C12, Neuro-2a, Rat spleen, Rat lung
Cellular Localization:
Cytoplasm, Nucleus, Secreted.
Calculated MW:
26kDa
Observed MW:
28kDa
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
3958
RRID
AB_2861687
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and Galectin 3/Galectin 3/LGALS3 knockout (KO) HeLa cells, using [KO Validated] Galectin 3/LGALS3 Rabbit pAb (CAB13506) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of C6 cells using [KO Validated] Galectin 3/LGALS3 Rabbit pAb (CAB13506) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using [KO Validated] Galectin 3/LGALS3 Rabbit pAb (CAB13506) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
