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[KO Validated] KRAS Rabbit Polyclonal Antibody (CAB12704)

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SKU:
CAB12704
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Synonyms:
NS, NS3, OES, CFC2, RALD, K-Ras, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras 2, 'C-K-RAS, c-Ki-ras, c-Ki-ras2, AS
Research Use:
Cell Biology
  • KO Validated Antibodies 1 Anti-KRAS Antibody CAB12704KO Validated
  • KO Validated Antibodies 1 Anti-KRAS Antibody CAB12704KO Validated
  • KO Validated Antibodies 1 Anti-KRAS Antibody CAB12704KO Validated
€113 - €457
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Description

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[KO Validated] KRAS Antibody (CAB12704)

The [KO Validated] KRAS Antibody (CAB12704) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in a rabbit host, is highly specific to human KRAS samples and has been validated for use in Western blot applications. By binding to the KRAS protein, this antibody allows for precise detection and analysis of KRAS expression in various cell types, making it essential for studies in cancer biology and precision medicine.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] KRAS Antibody
SKU:CAB12704
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:DEYD PTIE DSYR KQVV IDGE TCLL DILD TAGQ EEYS AMRD QYMR TGEG FLCV FAIN NTKS FEDI HHYR EQIK RVKD SEDV PMVL VGNK CDLP SRTV DTKQ AQDL ARSY GIPF IETS AKTR QRVE DAFY TLVR EIRQ YRLK KISK EEKT PGCV KIKK CII
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:1000 - 1:5000
IF/ICC1:50 - 1:200
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NS, NS3, OES, CFC2, RALD, K-Ras, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras 2, 'C-K-RAS, c-Ki-ras, c-Ki-ras2, AS
Positive Sample:Mouse brain, Rat brain
Cellular Localization:Cell Membrane, Cytoplasm, Cytoplasmic Side, Lipid-Anchor, Cytosol.
Calculated MW:22kDa
Observed MW:21kDa

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Purification MethodAffinity purification
Gene ID3845
RRIDAB_2861676
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and KRAS knockout (KO) 293T cells, using [KO Validated] KRAS Rabbit pAb (CAB12704) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
Product ImageWestern blot analysis of various lysates, using [KO Validated] KRAS Rabbit pAb (CAB12704) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.

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