The LCAT Antibody (CAB11741) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is specifically designed for use with human samples and has been validated for Western blot applications. By binding to the LCAT protein, this antibody allows for the detection and analysis of LCAT expression in various cell types, making it essential for studies in lipid metabolism, cardiovascular disease, and lipid-related disorders.LCAT, or lecithin-cholesterol acyltransferase, is a key player in the process of cholesterol esterification, playing a crucial role in reverse cholesterol transport and maintaining cholesterol homeostasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
LCAT Antibody
SKU:
CAB11741
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LCAT
Positive Sample:
Mouse brain
Cellular Localization:
Secreted.
Calculated MW:
50kDa
Observed MW:
60kDa
This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency.
Purification Method
Affinity purification
Gene ID
3931
RRID
AB_2758728
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from mouse brain, using LCAT Rabbit pAb (CAB11741) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from Human plasma, using LCAT Rabbit pAb (CAB11741) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
