The LIMA1 Antibody (CAB11682) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. RRID AB_2758685 Gene ID 51474 Swiss Prot Synonym EPLIN; LDLCQ8; SREBP3; LIMA1
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
LIMA1 Antibody
SKU:
CAB11682
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:100 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. RRID AB_2758685 Gene ID 51474 Swiss Prot Synonym EPLIN; LDLCQ8; SREBP3; LIMA1
Purification Method:
Affinity purification
Gene ID:
51474
RRID:
AB_2758685
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-549 cells, using LIMA1 Rabbit pAb (CAB11682) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
