The Monkey MCSF (Macrophage Colony-Stimulating Factor 1) ELISA Kit is a powerful tool for the accurate measurement of MCSF levels in monkey samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it an ideal choice for a variety of research applications.MCSF is a key factor in regulating the growth and differentiation of macrophages, playing a crucial role in the immune response and inflammation.
By measuring MCSF levels, researchers can gain valuable insights into the functioning of the immune system and its role in various diseases and conditions.Whether studying immune responses, inflammation, or cell differentiation, the Monkey MCSF ELISA Kit provides researchers with a valuable tool for understanding the role of MCSF in monkey samples. Trust in the accuracy and precision of this kit for your research needs.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey MCSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey MCSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey MCSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey MCSF. You can calculate the concentration of Monkey MCSF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
96-108
96-110
Average (%)
92
102
103
1:4
Range (%)
87-100
93-107
95-112
Average (%)
92
100
103
1:8
Range (%)
84-95
95-112
93-107
Average (%)
90
103
100
1:16
Range (%)
88-100
95-108
97-109
Average (%)
93
102
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
96
EDTA plasma (n=5)
92-108
100
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.13
242.57
996.98
100.14
237.73
949.78
Standard deviation
5.71
13.66
32.3
6.1
12.53
35.43
C V (%)
6.07
5.63
3.24
6.09
5.27
3.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey MCSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey MCSF in samples. No significant cross-reactivity or interference between Monkey MCSF and analogues was observed.
