Mouse Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) ELISA Kit
The Mouse Acetyl-Coenzyme A Acetyltransferase 2 (ACAT2) ELISA Kit is a specialized assay designed for the accurate and precise detection of ACAT2 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for your research needs.ACAT2 is a key enzyme involved in lipid metabolism, specifically in the synthesis of cholesterol and fatty acids. Dysregulation of ACAT2 has been linked to metabolic disorders such as atherosclerosis and non-alcoholic fatty liver disease, highlighting its importance as a potential biomarker for studying these conditions and developing therapeutic interventions.
With its easy-to-use format and robust performance, the Mouse ACAT2 ELISA Kit is a valuable tool for investigating the role of ACAT2 in metabolic pathways and exploring its potential as a target for drug development. Order your kit today and take your research to the next level.
Product Name:
Mouse Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
