Mouse BMP8B (Bone Morphogenetic Protein 8B) CLIA Kit
The Mouse BMP8B (Bone Morphogenetic Protein 8B) CLIA Kit is a highly reliable and sensitive assay designed for the precise measurement of BMP8B levels in mouse serum, plasma, and tissue homogenates. This kit provides accurate and reproducible results, making it an indispensable tool for researchers studying the role of BMP8B in various biological processes.BMP8B is a key member of the bone morphogenetic protein family, playing a crucial role in bone development, metabolism, and energy expenditure.
Dysregulation of BMP8B has been linked to obesity, diabetes, and other metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse BMP8B CLIA Kit offers a comprehensive solution for analyzing BMP8B levels in mouse samples, providing researchers with the necessary tools to advance their understanding of BMP8B's role in physiology and pathology.
Product Name:
Mouse BMP8B (Bone Morphogenetic Protein 8B) CLIA Kit
SKU:
MOES00126
Target:
Mouse BMP8B (Bone Morphogenetic Protein 8B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse BMP8B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP8B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BMP8B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse BMP8B. You can calculate the concentration of Mouse BMP8B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-110
98-110
97-114
Average (%)
104
104
104
1:4
Range (%)
93-105
92-107
90-100
Average (%)
100
99
95
1:8
Range (%)
97-113
90-101
84-97
Average (%)
103
95
90
1:16
Range (%)
97-113
102-116
100-115
Average (%)
104
109
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
96-111
103
Cell culture media (n=5)
101-119
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.06
53.54
190.09
25.65
57.65
178.28
Standard deviation
2.1
4.91
12.72
2.24
6.23
11.52
C V (%)
8.38
9.17
6.69
8.73
10.81
6.46
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse BMP8B concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse BMP8B in samples. No significant cross-reactivity or interference between Mouse BMP8B and analogues was observed.
