The Mouse CALP (Calprotectin) ELISA Kit is a powerful tool for detecting calprotectin levels in mouse biological samples, including serum, plasma, and tissue lysates. This kit boasts high sensitivity and specificity, providing researchers with reliable and consistent results for a variety of experimental applications.Calprotectin, a member of the S100 protein family, is an important biomarker of inflammation and is involved in various immune responses. Elevated levels of calprotectin have been associated with inflammatory bowel diseases, rheumatoid arthritis, and other inflammatory conditions, making it a valuable target for research and diagnostic purposes. With the Mouse CALP ELISA Kit, researchers can accurately measure calprotectin levels in their samples, providing valuable insights into the inflammatory processes at play in their experimental models. This kit is an essential tool for studying the role of calprotectin in immune responses and inflammatory diseases, offering a reliable and efficient way to assess calprotectin levels in mouse samples.
Product Name:
Mouse CALP (Calprotectin) ELISA kit
SKU:
MOES01549
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
