The Mouse Casp14 (Caspase-14) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of caspase-14 levels in mouse samples. This kit is ideal for the detection of this crucial enzyme in serum, plasma, and cell culture supernatants, providing researchers with reliable and reproducible results for a variety of research applications.Caspase-14 is a key player in apoptotic pathways, regulating cell death and inflammation. Dysregulation of caspase-14 activity has been implicated in various diseases, including cancer, inflammatory disorders, and skin diseases.
Therefore, measuring caspase-14 levels is essential for understanding its role in disease pathogenesis and potential therapeutic interventions.With its high sensitivity and specificity, the Mouse Casp14 CLIA Kit is a valuable tool for researchers studying apoptotic pathways and seeking to unravel the complex mechanisms underlying various diseases. Don't miss out on this essential kit for your research needs.
Product Name:
Mouse CASP14 (Caspase 14) CLIA Kit
SKU:
MOES00156
Target:
Mouse CASP14 (Caspase 14)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP14. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CASP14. You can calculate the concentration of Mouse CASP14 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
96-113
89-101
Average (%)
94
104
94
1:4
Range (%)
88-104
90-103
102-115
Average (%)
95
96
109
1:8
Range (%)
101-113
88-102
90-104
Average (%)
106
93
97
1:16
Range (%)
87-98
84-95
91-106
Average (%)
92
90
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
93
EDTA plasma (n=5)
92-102
97
Cell culture media (n=5)
94-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
205.62
356.97
1501.28
212.83
379.64
1527.76
Standard deviation
26.71
41.84
125.96
26.54
43.81
158.28
C V (%)
12.99
11.72
8.39
12.47
11.54
10.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CASP14 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CASP14 in samples. No significant cross-reactivity or interference between Mouse CASP14 and analogues was observed.
