The Mouse CLU (Clusterin) ELISA Kit is specifically designed for the precise and quantitative measurement of Clusterin levels in various biological samples. Clusterin, a versatile glycoprotein with diverse roles in cellular and physiological processes, is recognized for its involvement in numerous functions, including cell survival, apoptosis, lipid transportation, and regulation of the immune response. Accurate measurement of Clusterin is essential for gaining insights into its multifaceted functions within the body, particularly its implications in disease pathogenesis, neurodegenerative disorders, cancer progression, and renal dysfunction among other conditions. As a critical player in maintaining cellular homeostasis and responding to various stressors, assessing Clusterin levels provides valuable information for understanding its roles in health and disease. The Mouse CLU ELISA Kit by Assay Genie offers exceptional sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under strict quality control guidelines, this kit guarantees robust performance and ease of use, making it an ideal choice for researchers investigating the intricate functions of Clusterin in biological systems.
Product Name:
Mouse CLU (Clusterin) ELISA Kit
SKU:
AEES00259
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
