Description
Mouse Cytokine Array (34 targets) (SARB0082)
The Mouse Cytokine Array (SARb0082) from Assay Genie is a powerful tool for researchers studying cytokine expression in mouse samples. This array kit allows for the simultaneous detection of 34 different cytokines, providing comprehensive information on the immune response in a single experiment.With a simple and efficient protocol, the Mouse Cytokine Array kit offers high sensitivity and specificity, ensuring reliable and accurate results. This array is suitable for a variety of sample types, including serum, plasma, cell lysates, and tissue homogenates, making it versatile for different research applications.
Researchers can utilize this array to study cytokine profiles in various disease models, such as cancer, autoimmune diseases, and infectious diseases. The comprehensive panel of cytokines allows for a holistic view of the immune response, aiding in the discovery of potential biomarkers and therapeutic targets.Overall, the Mouse Cytokine Array (SARb0082) from Assay Genie is a valuable tool for researchers looking to explore the complex interactions of cytokines in immune regulation and disease pathogenesis.
| Product Sku: | SARB0082 |
| Size: | 2, 4, or 8 |
| Species Detected: | Mouse |
| Number of Targets Detected: | 34 |
| Gene Symbols: | CCL22, CXCL11, CXCL15, DPP4, FCGR2B, FGF2, FIGF, FLT1, FLT3LG, FLT4|ICAM1, IGF1, IGF2, IGFBP2, IL15, IL17RB, IL7, KDR, MET, MMP2, MMP3, MMP9, PPBP, RETN, SELE, SHH, SPP1, TIMP2, TNFRSF11B, TNFRSF18, TNFRSF19, TNFSF11, TSLP, TYRO3 |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
bFGF
| CD26 (DPPIV)
| Dtk
| E-Selectin
| Fc gamma RIIB (CD32b)
|
Flt-3 Ligand
| GITR (TNFRSF18)
| HGFR
| ICAM-1 (CD54)
| IGFBP-2
|
IGF-1
| IGF-2
| IL-15
| IL-17 RB
| IL-7
|
I-TAC (CXCL11)
| Lungkine (CXCL15)
| MDC (CCL22)
| MMP-2
| MMP-3
|
Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
| Pro-MMP-9
| Resistin
| Sonic Hedgehog N-Terminal (Shh-N)
|
TCK-1 (CXCL7)
| TIMP-2
| TRANCE (TNFSF11)
| TROY (TNFRSF19)
| TSLP
|
VEGFR1
| VEGFR2
| VEGFR3
| VEGF-D
| |
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Mouse Cytokine Antibody Array C4 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
