The Mouse E-Cad (E-Cadherin) CLIA Kit is specifically designed for the precise measurement of E-Cadherin levels in mouse samples, including serum, plasma, and tissue homogenates. With its high sensitivity and accuracy, this kit ensures reliable and consistent results for various research applications.E-Cadherin is a key protein that plays a critical role in cell adhesion and maintaining tissue integrity. Dysregulation of E-Cadherin has been linked to various diseases, including cancer and developmental disorders, highlighting its importance as a biomarker for studying these conditions.
With the Mouse E-Cad CLIA Kit, researchers can delve into the intricate mechanisms of cell adhesion and explore the potential implications of E-Cadherin in disease progression. Trust in this kit for robust and insightful results that can contribute to advancements in biomedical research.
Product Name:
Mouse E-Cad (E-Cadherin) CLIA Kit
SKU:
MOES00133
Target:
Mouse E-Cad (E-Cadherin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse E-Cad and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse E-Cad, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse E-Cad. You can calculate the concentration of Mouse E-Cad in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-114
102-118
98-115
Average (%)
108
110
106
1:4
Range (%)
103-119
90-100
97-113
Average (%)
110
95
105
1:8
Range (%)
93-109
91-105
101-118
Average (%)
101
97
109
1:16
Range (%)
92-106
88-101
88-100
Average (%)
97
95
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
105
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
102-116
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.98
84.0
358.52
45.95
76.8
383.64
Standard deviation
4.76
7.71
28.5
4.02
7.2
35.91
C V (%)
10.13
9.18
7.95
8.75
9.38
9.36
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse E-Cad concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse E-Cad in samples. No significant cross-reactivity or interference between Mouse E-Cad and analogues was observed.
