The Mouse EGFR (Epidermal Growth Factor Receptor) ELISA Kit is expertly crafted for the precise and quantitative measurement of EGFR levels in a variety of biological samples. EGFR, known for its crucial role as a transmembrane receptor tyrosine kinase, is a key player in diverse cellular processes such as cell proliferation, survival, differentiation, and migration. Signaling through EGFR regulates fundamental cellular functions and pathways, making it a significant target for research in various fields. Accurately measuring EGFR levels is essential for gaining insights into its involvement in growth factor signaling, cancer progression, and other physiological and pathological processes. Understanding the expression and activity of EGFR can provide valuable information for studying its impact on cell behavior, disease development, and therapeutic interventions. The Mouse EGFR ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Produced under rigorous quality control measures, this kit delivers robust performance and user-friendly features, making it an excellent choice for researchers investigating the functions and implications of Epidermal Growth Factor Receptor in biological systems.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
