The Mouse ENG (Endoglin) ELISA Kit is specifically designed for the precise measurement of endoglin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, making it a valuable tool for various research purposes.Endoglin is a key protein involved in angiogenesis and vascular development, playing a critical role in regulating blood vessel growth and remodeling. Dysregulation of endoglin has been linked to various diseases, including cancer, cardiovascular disorders, and inflammation, making it a significant biomarker for studying these conditions and exploring potential therapeutic interventions.With its user-friendly design and superior performance, the Mouse ENG (Endoglin) ELISA Kit is an essential tool for researchers investigating the role of endoglin in disease pathogenesis and exploring novel treatment strategies.
Product Name:
Mouse ENG (Endoglin) ELISA Kit
SKU:
MOES01486
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.