The Mouse ER Alpha (Estrogen Receptor Alpha) CLIA Kit is specifically designed for the precise detection of ER Alpha levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.ER Alpha is a key protein involved in mediating estrogen signaling, regulating gene expression, and influencing cell growth and differentiation. It plays a critical role in various physiological processes, including development, reproduction, and metabolism. Dysregulation of ER Alpha has been implicated in numerous diseases, such as breast cancer, osteoporosis, and cardiovascular disorders, highlighting its importance as a target for therapeutic interventions. The Mouse ER Alpha CLIA Kit provides researchers with a valuable tool for studying the role of ER Alpha in health and disease, enabling the exploration of new treatment strategies and diagnostic approaches. Its reliable performance and user-friendly format make it an ideal choice for laboratories conducting studies on estrogen receptor signaling pathways and related biological processes.
Product Name:
Mouse ER alpha (Estrogen Receptor Alpha) CLIA Kit
SKU:
MOES00250
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
